UbcH3; active 10 ug

Code: 23-022 D2-231

Application

Active, full length, recombinant human UbcH3. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.


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Your Price
€219.50 EACH
€269.99 inc. VAT

Application

Active, full length, recombinant human UbcH3. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

General description

Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or an Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate.
UbcH3 has been shown to interact with a number of different E3 ligases, including SCFSkp2/Cks1, promoting the ubiquitination of p27 and SCFβTrCP1, promoting the ubiquitination of β-catenin and IκBα.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

biological sourcehuman
manufacturer/tradenameUpstate®
NCBI accession no.NM_004359
Quality Level100
recombinantexpressed in baculovirus infected Sf21 cells
technique(s)activity assay: suitable
This product has met the following criteria to qualify for the following awards:



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